《植物生理学报》 2010, 46(10): 1033-1039

水稻MYB 转录因子OsDUO1 的cDNA 克隆及其表达模式初步分析

李素玲^1,2, 宗杰², 周志刚¹, David Twell³, 张大兵^2,*

1 上海海洋大学水产与生命学院, 上海 201306; 2 上海交通大学生命科学与技术学院, 上海 200240; 3Department of Biology, University of Leicester, United Kingdom LE17RH

通信作者：张大兵；E-mail: zhangdb@sjtu.edu.cn；Tel: 021-34205073

摘　要：

我们利用 RT-PCR 方法成功从水稻中克隆了 R2R3 类 MYB 转录因子OsDUO1 (Oryza sativa d uo pollen 1)的全长为1032 bp 的 cDNA, 该基因编码一个 343 个氨基酸残基的蛋白。RT-PCR 分析结果表明 OsDUO1 只在水稻的花粉发育后期 表达,说明 OsDUO1 可能对水稻花粉发育具有生物学功能。生物信息学分析表明, OsDUO1 在短柄草、高粱、玉米、拟南 芥、烟草、葡萄、蓖麻、杨毛果、小立碗藓植物中有相近同源序列, 暗示该基因在进化中具有保守的生物学功能。

关键词：水稻; OsDUO1; 克隆; 表达模式; 进化分析

收稿：2010-09-01   修定：2010-09-14

资助：国家自然科学基金项目(2009CB941500)。

Cloning and Expression Analysis of OsDUO1 Encoding a Rice MYB Transcription Factor

LI Su-Ling^1,2, ZONG Jie², ZHOU Zhi-Gang¹, David Twell³, ZHANG Da-Bing^2,*

¹College of Fisheries and Life, Shanghai Ocean University, Shanghai 201306, China; ²School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China; ³Department of Biology, University of Leicester, United Kingdom LE17RH

Corresponding author: ZHANG Da-Bing; E-mail: zhangdb@sjtu.edu.cn; Tel: 021-34205073

Abstract:

We successfully cloned the full cDNA sequence of OsDUO1 (Oryza sativa duo pollen 1) encoding a putative R2R3 type MYB transcription factor by RT-PCR. The OsDUO1 cDNA is 1 032 bp in length and encodes a protein with 343 amino acids. The expression of OsDUO1 was detectable only during the late pollen development in rice, suggesting the possible biological function of OsDUO1 during late rice pollen development. Bioinformatics analysis showed that OsDUO1 has close homologs in Brachypodium distachyon, Sorghum bicolor, Zea mays, Arabidopsis thaliana, Nicotiana tabacum, Vitis vinifera, Ricinus communis, Populus trichocarpa and Physcomitrella patens suggesting the conserved function of OsDUO1 in plants during evolution.

Key words: rice; OsDUO1; clone; expression pattern; phylogenetics analysis